Figure 1. Bcl6, CD25 and TIGIT levels in various conventional and regulatory T cell subsets.

Splenic CD4+ T cells from WT mice immunized with Sheep Red Blood Cells (SRBC) 7 d before were studied by flow cytometry. (A) Foxp3+ and Foxp3- CD4+ T cells are gated as in the flow plot (left). In Foxp3- conventional CD4+ T (Tconv) cells, Tfh cells, PD-1+TH cells and PD-1- TH cells are defined as CXCR5^hiPD-1^hi, CXCR5^negPD-1⁺ and CXCR5^negPD-1^neg, respectively. In Foxp3+ regulatory CD4+ (Treg) cells, Tfr, PD-1+Treg and PD-1- Treg cells are gated on CXCR5^hiPD-1^hi, CXCR5^-PD-1⁺ and CXCR5^-PD-1^-, respectively (right). Bcl6 (B), CD25 (C) and TIGIT (D) mean fluorescent intensity (MFI) of Tfh, PD-1+TH, PD-1- TH, Tfr, PD-1+Treg and PD-1- Treg cells. Data shown as mean +/− SEM, n = 4-5. *p < 0.05, **p < 0.01, *** p < 0.001 (ANOVA). Data are representative of two independent experiments with similar results.