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. 2016 May 11;16(Suppl 1):35. doi: 10.1186/s12896-016-0261-1

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© Rahman et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Southern hybridization analysis of EcNAC67transgenic rice (ASD16)linesin T₀ generation; where + ve control is a linearized plasmid (pCAMBIA1300 + ECNAC67), −ve control is Non-transgenic ASD16 and EcNAC67 (E1 to E6) are independent transgenic events; A and B represents two different transgenic plants of respective lines; About 20ug of genomic DNA was digested with BamHIand hybridized using Hygromycin (hpt) probe