Fig. 7.

Elevated intracellular basal Ca²⁺ signal in dysferlin-mutated myotubes is reduced by Cx HC inhibitor. Four different dysferlin-mutated myotubes (called 107, 379, AB320 and ER) were used, which were differentiated to myotubes with differentiation medium (DMEM/F12 supplemented with 5 % horse serum plus insulin 10 ug/ml). The Ca²⁺ signal was evaluated using the FURA-2 AM dye in a fluorescence microscope. Signals were compared to those obtained in control myotubes (RCMH). Molecule D4 (D4, 100 nM), a selective connexin hemichannels (Cx HC) inhibitor, was used. During the last 7 days (D4 was added daily during 7 days) before the Ca²⁺ measurement was performed, D4 was applied to prevent the increase of intracellular basal Ca²⁺ signal in human dysferlin mutated myotubes. The data represent as mean ± SEM. ANOVA test with Dunn post test. *P < 0.05. n = 4 cell cultures for each cell line