Skip to main content
. Author manuscript; available in PMC: 2016 Jun 7.
Published in final edited form as: Leuk Lymphoma. 2010 Sep;51(9):1734–1747. doi: 10.3109/10428194.2010.501535

Figure 5.

Figure 5

Arsenic Trioxide enhances ATRA induced differentiation of HL-60 cells. Panel A shows the percentage of cells (y-axis) with capability for inducible oxidative metabolism at the indicated time points. Cells were treated with TPA to induce oxidative metabolism. Using a functional marker of differentiation, i.e. generation of Reactive Oxygen Species (ROS) we measured terminal differentiation in untreated cells, cells treated with ATRA, a combination of ATRA-ATO, and ATO alone. The percentage of positive cells with TPA, when gates were set to exclude 95% of control cells (without TPA), is shown. There was no statistically significant difference in the percentage of differentiated cells in the ATRA alone versus ATRA-ATO combination treatments at 48h. At 72h, using a one-tailed test we concluded that percentage of DCF positive cells was less in the ATRA case when compared with the ATRA-ATO combination case with a 90% confidence level (p=0.0953). The background ROS activity in all the samples was measured by cells treated with DMSO.