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. Author manuscript; available in PMC: 2016 Jun 7.
Published in final edited form as: Leuk Lymphoma. 2010 Sep;51(9):1734–1747. doi: 10.3109/10428194.2010.501535

Figure 7.

Figure 7

ATRA abrogates the apoptotic effect of ATO in HL-60 cells. Western analysis of cleaved and uncleaved PARP at 24 and 48 h is shown here. The different lanes are control (untreated) cells, and cells treated with ATRA, combination of ATRA-ATO, and ATO alone. Cleavage of PARP is an indicator of apoptosis. The figure shows that we saw some progress towards apoptosis in the ATO alone treatment. However, the combination treatment of ATRA-ATO or the ATRA alone treatment did not show cleavage of PARP. This result suggests that ATRA rescues the cells from apoptotic effect of ATO, and that there is no increase in apoptotic percentage of cells in the combination treatment. Loading control is shown by levels of GAPDH at 24h and 48h.