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. Author manuscript; available in PMC: 2016 Jun 7.
Published in final edited form as: Dev Cell. 2016 Mar 21;36(6):611–623. doi: 10.1016/j.devcel.2016.02.024

Figure 1. A functional screen to match KRAB-ZFPs repressors with their genomic targets.

Figure 1

(A) Methodological outline. A library of murine KRAB-ZFPs-expressing plasmids was transfected in 293T stable cell lines containing the DNA sequence of interest upstream a PGK-GFP cassette. Fluorescence readout in 96-well plates was normalized for protein content, and hits were tested by FACS for confirmation. (B) Validation of the screen using the previously characterized ZFP809/PBSPro (Pro) pair. Fluorescence readout was performed (left) and identified hits were tested by FACS. The only hit confirmed by FACS was ZFP809 (right panel). Black: transfection control; red: hits identified by plate reader; light red: hits confirmed by FACS. Error bars represent SD, ***p < 0.001, Student’s t test. See also Figure S1, Table S1, and Table S2.