Fig. 2.

TM-FT image procedure. (a) During step 1, conventional FT is performed using an eight-source and eight-detector configuration. (b) Low-resolution map of μ_af is then obtained using the standard FT reconstruction algorithm. This low-resolution map is used to determine the ROI for the HIFU heating in the second step. (c) A specific source-detector pair is selected with the best fluorescence signal in terms of SNR and sensitivity. The HIFU beam scans through the ROI determined from the previous step. This step provides a spatial a priori on the localization of the fluorescent source μ_af. (d) This a priori information is then used in the TM-FT image reconstruction algorithm to obtain a high-resolution image with superior quantitative accuracy.