Figure 2.

Immunoblot analysis of ABC transporters P-gp (a), MRP1 (b), MRP4 (c) and BCRP (d) in testicular tissue lysates from five uninfected and five HIV-1-infected, treated subjects. Protein lysates from cell lines overexpressing P-gp, MRP1 and MRP4 were loaded in the first lane and used as positive controls (+) for their respective immunoblots while the breast cancer cell line MX100, known to overexpress BCRP, was used as the positive control (+) for BCRP. β-Actin was used as the loading control for each immunoblot. Densitometric analysis results are shown alongside their respective immunoblots. Differences between the two experimental groups were not statistically significant (P > 0.05).