Figure 3.

Immunoblot analysis of SLC transporters OATP2B1 (a), OAT1 (b), CNT1 (c) and ENT2 (d) in testicular tissue lysates from five uninfected and five HIV-1-infected, treated subjects. Protein lysates isolated from the MDCK-OATP2B1 cell line were used as the positive control (+) for OATP2B1, whereas HepG2 cell protein lysate was used as the positive control (+) for OAT1, CNT1 and ENT2. β-Actin was used as the loading control for each immunoblot. Densitometric analysis results are shown alongside their respective immunoblots. Differences between the two experimental groups were not statistically significant (P > 0.05).