Table 1. Properties of bioinformatically identified candidate antigens and previously identified antigens.
| T.annulata_id | T.parva_id | dN | dS | dN/dS value | Protein identity | Nucleotide identity | Macro stage | Mero stage | Piro. stage | chr. location | Product | SignalP | TMHMM | GPI anchor | NLS | Protein info. (putative) | Conserved for Theileria | fMotif | fDomain info. (number) | Gene ontology (molecular function) | Apicoplast targeting | Rhoptry/microtubules | Macro EST hits | Mero EST hits | Piro EST hits | Mass (kDa) | Immunogenicity |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TA06510*a | TP01_0939 | 0.23 | 1.43 | 0.16 | 63.5 | 72.9 | 1 | 0 | 0 | 1 | hypothetial protein | √ | 1 | √ | - | - | - | - | F(1) | - | - | - | 11 | 0 | 0 | 37 | ++ |
| TA20440*a | TP01_0522 | 0.31 | 0.76 | 0.40 | 57.1 | 72.6 | 1 | 1 | 0 | 1 | hypothetical membrane protein (TaSP alike) | √ | 3 | - | - | - | - | - | F(1) | - | √ | - | 2 | 6 | 0 | 28 | ⫠ |
| TA13755*a | TP02_0543 | 0.29 | 0.88 | 0.32 | 59.6 | 71.3 | 1 | 1 | 0 | 2 | hypothetical prot. (TaSP alike) | √ | 3 | - | - | - | - | - | F(1) | - | √ | - | 6 | 2 | 0 | 53.5 | ⫠ |
| TA15040* | TP02_0777 | 0.22 | 1.02 | 0.21 | 69.2 | 74.6 | 1 | 0 | 0 | 2 | hypothetical prot., conserved | √ | 3 | - | - | - | - | - | F(1),DH | Zinc ion binding | √ | - | 20 | 0 | 0 | 25.5 | nd |
| TA15705a,c | TP02_0895 | 0.33 | 1.05 | 0.32 | 58.9 | 68.7 | 1 | 0 | 0 | 2 | hypothetical protein (Ta9) | √ | - | - | - | secreted | - | - | - | - | - | - | 10 | 0 | 0 | 35 | ++++ |
| TA15710a,d | TP02_0896 | 0.47 | 1.34 | 0.35 | 47.4 | 63.0 | 1 | 0 | 0 | 2 | hypothetical protein | √ | - | - | - | secreted | - | - | - | - | - | - | 4 | 0 | 0 | 38 | ++++ |
| TA15685a,d | TP02_0890 | 0.34 | 0.89 | 0.38 | 54.8 | 70.4 | 0 | 0 | 0 | 2 | hypo. protein | √ | - | - | - | secreted | - | - | F(1) | - | - | - | 0 | 0 | 0 | 28.1 | +++ |
| TA15690a,d | - | - | - | - | - | - | 1 | 0 | 0 | 2 | hypo. protein | √ | - | - | - | secreted | - | - | - | - | - | - | 10 | 0 | 0 | 35.9 | +++ |
| TA15695a,d | TP02_0888 | 0.77 | 20.9 | 0.04 | 29.5 | 51.4 | 1 | 0 | 0 | 2 | hypo. protein | √ | - | - | - | secreted | - | - | - | - | - | - | 17 | 0 | 0 | 18.8 | ++ |
| TA11610*a | TP02_0148 | 0.01 | 0.85 | 0.01 | 97.8 | 88.0 | 1 | 1 | 1 | 2 | heat shock 70 protein | - | - | - | 1 | - | - | - | - | ATP binding | - | - | 13 | 31 | 25 | 71 | ⫠ |
| TA17315a,b | TP04_0051 | 0.31 | 1.28 | 0.24 | 59.9 | 68.5 | 1 | 1 | 0 | 4 | surface protein precursor (TaSP) | √ | 3 | - | - | integral membrane | - | P | F(1) | Copper ion memb. transp | - | - | 11 | 7 | 0 | 35.5 | ++++ |
| TA17050b,e | TP01_1056 | 0.2 | 0.72 | 0.28 | 72.9 | 77.4 | 0 | 1 | 1 | 1 | mero/piro surf. antigen (Tams) | √ | 1 | √ | - | mero. antigen | - | - | F(1) | - | - | - | 0 | 10 | 12 | 32.3 | +++ |
| TA03755b,e | TP03_0287 | 0.43 | 1.32 | 0.33 | 49.9 | 63.6 | 0 | 0 | 0 | 3 | sporozoite surface antigen (SPAG) | √ | 1 | √ | - | sporo. p67 surf. antigen | - | - | - | - | - | - | 0 | 0 | 0 | 93 | ⫠ |
| TA08425b,e | TP04_0437 | 0.34 | 1.15 | 0.29 | 58.2 | 67.6 | 1 | 1 | 0 | 4 | T.parva microneme-rhoptry antigen (Tamr-1) | √ | 1 | √ | 1 | - | 1 | - | F(4) | - | - | 1 | 1 | 2 | 0 | 101.9 | nd |
| TA13810e | TP02_0551 | 0.09 | 0.59 | 0.16 | 83.4 | 84.6 | 0 | 1 | 1 | 2 | ts-chitose type piroplasm surf-like protein (MeroI) | √ | 1 | √ | - | - | - | - | F(1) | - | - | - | 0 | 9 | 2 | 26.8 | + |
| TA20980a | TP01_0380 | 0.44 | 2.81 | 0.16 | 51.2 | 62.2 | 1 | 1 | 0 | 1 | proline-rich hypo. prot. (NC1) | √ | - | - | 1 | - | - | - | - | - | - | - | 2 | 4 | 0 | 111.1 | nd |
| TA16090a | TP01_0966 | 0.42 | 1.57 | 0.26 | 51.8 | 63.9 | 1 | 0 | 0 | 1 | glutenin, putative (NC10) | - | - | - | - | - | - | - | - | - | - | - | 6 | 0 | 0 | 119.5 | nd |
| TA03155e | - | - | - | - | - | - | 0 | 0 | 0 | 1 | Tash1-like protein | √ | 1 | - | 1 | Tash AT-hook prot. | - | P, D | - | - | - | - | 0 | 0 | 0 | 35.7 | ⫠ |
| TA10720e | TP04_0646 | 0.06 | 0.91 | 0.07 | 87.6 | 84.8 | 0 | 1 | 1 | 4 | HSP90 homologue | √ | 1 | - | - | - | - | H | ATP binding | √ | - | 0 | 2 | 1 | 104.2 | - | |
| TA06470e | TP01_0934 | 0.11 | 2.61 | 0.04 | 82.3 | 76.5 | 0 | 1 | 1 | 1 | chaperone prot. (HSP90 homologue) | √ | - | - | 1 | - | P | H | ATP binding | - | - | 0 | 1 | 7 | 115.7 | - | |
| TA16025e | TP02_0955 | 0.47 | 1.16 | 0.40 | 43.2 | 65.6 | 1 | 0 | 0 | 2 | PQ-rich, SVSPf | √ | - | - | - | Tash AT-hook prot. | - | P, T | - | - | - | - | 4 | 0 | 0 | 64.5 | - |
| TA17485e | TP01_1225 | 0.48 | 1.53 | 0.31 | 44.9 | 62.4 | 1 | 1 | 0 | 1 | SVSPf | √ | - | - | - | 1 | - | F(1) | - | - | - | 1 | 1 | 0 | 47.5 | - |
* bioinformatically identified genes encoding candidate antigenic proteins in the T. annulata genome sequence based on possession of SignalP, GPI anchor, TMHMM, dN/dS values and EST data.
a genes which were cloned and expressed as recombinant proteins to evaluate immunogenic properties.
b previously identified antigens and genes encoding antigenic proteins presumed to be immunogenic.
c gene encoding protein which was identified by immunoprecipitation and mass spectrophotometric analysis.
d TA15705 (Ta9) paralogue family proteins.
e recombinant proteins expressed from available constructs.
f used to indicate the abbreviations used for the FAINT (F), DHHC-type (DH), Histidine kinase-like ATPases (H) domains and PEST (P), DNA binding (D), TasHN (T) motifs.
nd immunogenic property of the proteins identified in previous studies.
T.annulata_id and T.parva_id; indicates GeneDB (http://www.genedb.org) accession numbers of each gene.