Table 1. The results of analytical gel filtration and the determination of the oligomeric status of each truncated fragments.
| UDE constructs | Tm (°C) | Analytical gelfiltration | Oligomer status | ||
|---|---|---|---|---|---|
| Vel (kDa) | Mapp (kDa) | Mcalc (kDa) | |||
| NA1 | 51,3 | 62,4 | 42,1 | 25,7 | dimer |
| NA2 | 53,9 | 56,6 | 57,0 | 26,3 | dimer |
| NA3 | 57,1 | 69,6 | 28,9 | 18,0 | dimer |
| NC6 | 53,7 | 55,7 | 35,6 | 34,7 | monomer |
| NG1 | 56,4 | 60,0 | 47,7 | 23,3 | dimer |
| NG3 | 51,5 | 64,6 | 37,5 | 20,9 | dimer |
| CA7 | - | 44,3 | - | 25,4 | monomer |
| CH9 | - | 58,3 | 52,1 | 22,1 | dimer |
| CH10 | - | 56,0 | 58,8 | 32.1 | dimer |
| full length UDE | monomer | ||||
Vel: elution volume, Mapp: apparent molecular weight, Mcalc: calculated molecular weight based on column calibration. Data of thermostability analysis by Thermofluor. Tm: melting point of the given protein.