Table 3. Values for multiple components of inhibition by L-glutamine and t-Pro of [3H]L-serine and [3H]D-serine transport into rat hippocampal astrocyte cultures.
[3H]D-serine Transport | Component 1 IC50 μM | Component 2 IC50 μM | Component 1 (%) | N |
L-glutamine | 26.0±2.8 | 2494±320 | 35.1±0.02 | 3 |
t-Pro | 23.9±9.4 | 2869±1463 | 60.6±0.06 | 3 |
[3H]L-serine Transport | Component 1 IC50 μM | Component 2 IC50 μM | Component 1 (%) | N |
L-glutamine | 30.3±11.3 | 3030±691 | 41.7±4.0 | 7 |
t-Pro | 21.3±4.9 | 2498±299 | 46.0±4.8 | 8 |
Components 1 and 2 were defined by the two-site curve-fitting program in GraphPad Prism. Values are the mean ± SEM of n observations. t-Pro is L-trans-4-hydroxyproline.