Fig 1. Binding of AURKA tetramer by transduced CD8+ effector cell populations.
CD8+ cells from three donors were transduced with siAUK or coAUK vectors, at a range of MOIs (vector supernatant applied to Retronectin-coated plates at 25%, 50%, and 100% of usual volume, diluted where appropriate with fresh culture medium). Binding of fluorophore-conjugated AUK207-215/HLA-A*0201 tetramer was analysed by flow cytometry. Genomic DNA was also collected, and vector copy number determined by qPCR. (A) Tetramer-binding and vector copy number were compared for the population as a whole. Correlation coefficients were compared using Preacher’s calculation (p<0.05). (B) Cells were also labelled for CD8+, and Vβ12 transgene expression. Cells from each MOI sample were gated for CD8 and Vβ12 positivity, and the percentage of tetramer-binding cells was recorded.