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. 2016 Jun 7;11(6):e0156896. doi: 10.1371/journal.pone.0156896

Fig 2. Sensitivity and Cytotoxicity by CD8+ cells in response to peptide-loaded target cells.

Fig 2

CD8+ cells were transduced with siAUK or coAUK vectors, and enriched on the basis of Vβ12 expression. NGM cells were used as a negative control. (A) To measure CD107a degranulation, C1R-A2 cells were pulsed with the indicated concentration of AUK207-215 peptide. Effector cells were co-cultured with target cells at E:T = 2:1. CD107a-APC antibody was added prior to 3 hours co-culture. CD107a labelling was assessed by FCM, and is presented as a percentage of CD8+ cells. For Chromium-release assessment of total cell killing, TCR-enriched T cells were co-cultured at a range of E:T ratios for 4 hours with Cr51-labelled, C1R-A2 target cells that were pulsed (B) or not pulsed (C) with 0.1μM AUK207-215 (* p<0.05, One-way ANOVA with Tukey’s test, n = 3, ± S.D.).