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Bulk siAUK or coAUK cells (ie. non-enriched) were stimulated weekly with peptide-pulsed C1R-0201 cells at E:T = 2:1. (A) Tetramer-binding was assessed by labelling with a fluorophore-conjugated AUK207-215 tetramer, and expressed as a percentage of Vβ12+ CD8 cells. (B) CD107a degranulation was assessed in response to stimulation, and the number of CD107a-positive cells was expressed as a percentage of Vβ12+ CD8 cells.
