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. 2016 Jun 7;11(6):e0156980. doi: 10.1371/journal.pone.0156980

Fig 1. Imprinted DNA methylation profiles of control blastocysts.

Fig 1

Black dots represent methylated CpGs, white dots represent unmethylated CpGs. The SNP location is indicated by an arrowhead. “MAT” represents the maternal oocyte-contributed allele; “PAT” represents the paternal sperm-contributed allele. A) Diagram of the SNRPN ICR amplified region analyzed on human chromosome 15q11.2 (GenBank: U41384) in control blastocysts, a 371 bp region consisting of 24 assessable CpGs and a G/A SNP (Rs220029). The SNRPN ICR is maternally methylated. Approximately 50% methylation is expected, derived from one MAT allele and one PAT allele. B) Diagram of the H19 ICR amplified region analyzed on human chromosome 11p15.5 (GenBank: AF087017) in control blastocysts, a 170 bp region consisting of 14 assessable CpGs and a A/C SNP (Rs2071094). The H19 ICR is paternally methylated. Approximately 50% methylation is expected, derived from one MAT allele and one PAT allele. C) Diagram of the KCNQ1OT1 ICR amplified region analyzed on human chromosome 11p15.5 (GenBank: U90095) in control blastocysts, a 265 bp region consisting of 22 assessable CpGs and a G/A SNP (Rs56134313). The KCNQ1OT1 ICR is maternally methylated. Approximately 50% methylation is expected, derived from one MAT allele and one PAT allele. D) Summary chart of percent methylation in all control blastocysts at the SNRPN (n = 20), H19 (n = 20), and KCNQ1OT1 (n = 20) ICRs. Each white diamond represents the methylation percentage for one individual control blastocyst per ICR. Average percent methylation for each cohort is indicated above each gene. Methylation dot diagrams for individual control blastocysts can be found in S1 Fig, and a summary can be found in S2 Table.