Table 2. PCR primers and parameters for bisulfite mutagenesis.

Locus	Gene	Primer Name	Primer Sequence (5’-3’)	Size (bp)	CpG (#)	SNPs	REF
15q11.2	SNRPN	SNRPN-OF	TAGTGTTGTGGGGTTTTAGGG	371	24	Rs220029	[27]
	(U41384)	SNRPN-IF	AGGGAGGGAGTTGGGATTT			G (84.8%) /
		SNRPN-IR	CACAACAACAAACCTCTAAACATTC			A (15.2%)
		SNRPN-OR	TACCCACCTCCACCCATATC
11p15.5	H19	H19-OF	AATAATGAGGTGTTTTAGTTTTATGGATG	170	14	Rs2071094	[27, 29]
	(Af087017)	H19-IF	TTGGTTGTAGTTGTGGAAT			C (66.4%) /
		H19-IR	ACTCCTATAAATATCCTATTCCCAAATAACCCC			A (33.6%)
		H19-OR	ACTTAAATCCCAAACCATAACACTAAAAC
11p15.5	KCNQ1OT1	KCNQ1OT1-OF	TGTTTTTGTAGTTTATATGGAAGGGTTAA	265	22	Rs56134313	[29, 30]
	(U90095)	KCNQ1OT1-IF	GTTAGGGAAGTTTTAGGGTGTGAAT			G (94.0%) /
		KCNQ1OT1-IR	AAACATACCAAACCACCCACCTAACAAA			A (6.0%)
		KCNQ1OT1-OR	CTCACCCCTAAAAACTTAAAACCTC

OF = outer forward primer, IF = inner forward primer, IR = inner reverse primer, OR = outer reverse primer

94°C for 10 min, 55 cycles of (94°C for 15 sec, 56°C for 20 sec, 72°C for 20 sec), 72°C for 10 min. [27]