Table 3. Expression primers and PCR parameters.

Locus	Gene	Primer Name	Primer Sequence (5’-3’)	Size (bp)
15q11.2	SNRPN	SNRPN-F	GCCATATTGGAGTAGCGAGGAA	84
	(NM_003097.3)	SNRPN-R	CAATGCAAGCTGGGCAGAA
	UBE3A	UBE3A-F	CAAAAATGGCCCAGACACAGA	119
	(NM_000462.3)	UBE3A-R	ACGTGATGGCCTTCAACAATC
11p15.5	H19	H19-F	GGAGTGAATGAGCTCTCAGG	308
	(NR_002196.2)	H19-R	CTAAGGTGTTCAGGAAGGCC
	KCNQ1OT1	KCNQ1OT1-F	AAAAGCTCCCAACGAGGAA	64
	(NR_002728.3)	KCNQ1OT1-R	GGCTCTTGGCTGGGTACA
	CDKN1C	CDKN1C-F	GGCCTCTGATCTCCGATTTC	67
	(NM_000076.2)	CDKN1C-F	ACATCGCCCGACGACTTC
7p13	PPIA	PPIA-F	GCTTTGGGTCCAGGAATGG	59
	(NM_001300981.1)	PPIA-R	TTGTCCACAGTCAGCAATGG

F = forward primer, R = reverse primer.

95°c for 10 min, 40 cycles of (95°C for 15 sec, 60°C for 1 min), 95°C for 15 sec, 60°C for 1 min, 95°C for 15 sec, 60°C for 15 sec