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. Author manuscript; available in PMC: 2016 Jun 7.
Published in final edited form as: J Neurochem. 2009 Sep 24;111(5):1202–1212. doi: 10.1111/j.1471-4159.2009.06404.x

Fig. 5.

Fig. 5

Pitx3 specifically binds to putative binding motifs in the 5′ upstream promoter of the vesicular monoamine transporter 2 (VMAT2) and dopamine transporter (DAT) gene. (a, e) Seven and five putative Pitx3-binding sites are identified in the 5′ promoter region of the VMAT2 and DAT genes, respectively. (b, f) Their nucleotide sequences are shown in 5′ to 3′ directions. (c, g) Chromatin immunoprecipitation analysis indicates that Pitx3 interacts with both VMAT2 and DAT gene promoters in vivo. The protein–DNA complexes were immunoprecipitated using antibodies against anti-HA. As a negative control, rabbit IgG was used. (d) Comparison of nucleotide sequences flanking the site 2 of the VMAT2 gene shows that they are highly conserved between mouse and rat.