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. Author manuscript; available in PMC: 2016 Jun 7.
Published in final edited form as: Nat Commun. 2015 Nov 27;6:8991. doi: 10.1038/ncomms9991

Figure 6. Type I IFN alters affinity conformation of CD11b.

Figure 6

(a) Shown are mean fluorescent intensities (MFI, ± S. D.) of CD11b expression on B-1a cells in pleural (top left) and peritoneal cavity B-1 cells (top right), PLN and MedLN (bottom left and right, respectively) from CD19/IFNAR−/− (open bars) mice and littermate controls (closed bars) at indicated times after infection with A/Mem71. (b) Adhesion assay showing fold-inductions of B-1 cell adhesion over media controls in vascular mimetic chambers under shear-flow when stimulated in vitro for 16h with IFNβ, or immediately prior with fMLP in the presence or absence of lovastatin, an inhibitor of integrin-activation. Cells were from wildtype (filled bars) or CD19/IFNAR−/− mice (open bars). (c) As in b, but pleural cavity B-1 cells were isolated from non-infected or day 2-A/Mem/71-infected mice prior to exposure to lovastatin. Student’s t-test: n.d., not done. n.s., not significant; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Data from one experiment (a,b,c) with n = 3 (a) or n = 12 pooled (b,c) mice per group.