Figure 2. The changing affinities certain monoclonal antibodies (mAbs) to immobilized HBsAg as a function of DTT concentration (unit: mM) during plate coating. (A) The binding profiles for 2 representative mAbs - 5F11 (highly sensitive for DTT treatment), 42B6 (insensitive to DTT treatment), with different DTT concentrations during plate coating. (B) Relative EC50 values of 22F10, 5F11, 15D1, A2C1, 127D7, and 42B6 as a function of DTT concentration. All 5 disulfide-sensitive mAbs lost most of their binding capacities to HBsAg with as low as 0.005 mM DTT during plate coating.