Skip to main content
. 2014 Feb 5;10(4):1013–1023. doi: 10.4161/hv.27753

Table 1. Characteristics of a panel of anti-HBsAg monoclonal antibodies.

Antibody Subclass Degree of disulfide sensitivitya Epitope typeb Dimer HBsAgc Monomer HBsAgc Binding strengthd EC50 (ng/mL)e
Highly sensitive to DTT treatment (>10)
22F10 IgG1       >440 C       -       - very strong 0.18
A2C1 IgG1       >240 C       -       - very strong 0.34
15D1 IgG1       >220 C       -       - very strong 0.36
5F11 IgG2a       >220 C       +       +/− very strong 0.37
127D7 IgG1       >33.5 L       +       + strong 2.4
20A2 IgG2b       10.1 C       -       +/− strong 2.9
Sensitive (1.3~10)
SF IgM       >9.02 C       +/−       - medium 8.9
A10C2 IgG1       6.74 L       +       + very strong 0.59
SA1 IgG2a       >3.63 C       -       - weak 22.0
E2A9 IgG2a       3.22 L       +       + strong 1.1
129G1 IgG1       2.01 L       +       + very strong 0.91
6C10 IgM       1.43 L       +       + strong 1.4
Not sensitive (0.7~1.3)
13H10 IgM       1.21 L       +       + medium 7.5
A13A2 IgG2b       0.97 L       +       + strong 2.2
42B6 IgG1       0.89 L       +       + very strong 0.94
G12F5 IgG1       0.77 L       +       + very strong 0.75
75C12 IgG1       0.72 L       +       + weak 29.8
Preferring reduced HBsAg (<0.7)
E7D4 IgG2a       0.49 L       +       + medium 4.2
E11E4 IgG2a       0.39 L       +       + strong 1.2
83H12 IgG1       0.23 L       +       + medium 4.6
45E9E IgG3       0.22 L       +       + weak 56.8
E9B3 IgG3       0.12 L       +       + weak 34.4

The degree of sensitivity to DTT treatment for a given mAb was indicated by fold change in EC50 value in direct binding ELISA. aDegree of disulfide sensitivity was assessed in a direct binding ELISA on HBsAg-coated plates. Relative binding data were derived from (DDT-treated HBsAg EC50)/ (HBsAg in PBS EC50) based on curve fitting results. bEpitope type identified via Western Blotting,15 “C” means conformational and “L” means linear. cMonomer HBsAg and dimer HBsAg15 were used by SIA (strip immunoblot assay), SIA strips contained 2 individual bands: a SDS-treated HBsAg dimer (5 μg, obtained from SDS-treated HBsAg by electro-elution), a SDS-treated HBsAg monomer (5 μg, obtained from SDS-treated HBsAg by electro-elution). dBinding strength for a given mAb was assigned based on the EC50 value of direct binding ELISA data. These mAbs were classified into 4 different groups: very strong (EC50 < 1 ng/mL), strong (1 ng/mL < EC50 < 3 ng/mL), medium (3 ng/mL < EC50 < 10 ng/mL), weak (10 ng/mL < EC50). eEC50 values were derived from 4-parameter logistics fits of the direct binding ELISA data. Binding affinity as reflected EC50 values may not correlate to the neutralization activity for different mAbs. The neutralization activity is more dependent on the epitope recognized by a given mAb.