Figure 2. The immune stimulation effect of CTB identified on BMDCs at multiplex levels. (A) Cytokines and chemokines induced 24 h after exposure to CTB. BMDCs were stimulated with either medium or 1μg/mL CTB. Supernatant was harvested 24 h later and analyzed for the presence of cytokines and chemokines according to the manufacturers’ instructions. Expression levels elevated by more than 1.5-fold relative to control were identified as upregulated. Moreover, the time-course and quantitative analysis of cytokines secreted by BMDCs post adjuvants stimulation was evaluated. The concentration levels of IL-6 (B), IL-10 (C), and IL-12 (D) at different time points were evaluated using quantitative ELISA, data from triplicate cultures are shown as mean ± SD *indicates significant difference (P < 0.05) when comparison CTB used with negative or positive control groups.