Figure 1. Production and characterization of recombinant Bet v 1 by Streptococcus thermophilus DSM 20617. (A) Map of vector pGh-Betv1; eryR, erythromycin resistance gene from Enterococcus faecalis; repA, plasmid replication protein; PT5, phage T5 promoter. (B) Western blot analysis of total cells extracts (30 µg of protein content) from rBet v 1-producing E. coli (lane 1) and Streptococcus thermophilus DSM 20617 (lane 2), and from wild type DSM 20617 (lane 3) detecting the 15 kDa rBet v 1 protein band.