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. 2016 May 16;113(22):6194–6199. doi: 10.1073/pnas.1602878113

Fig. S6.

Fig. S6.

Fig. S6.

Fig. S6.

Examples of Cy5-SSB being pushed by ssDNA translocases and diffusing freely on (dT)140. (A) Sample pushing FRET peaks for Pif1 pushing Cy5-SSB on 3′dT-Cy3-(dT)140 at 5 mM ATP. (B) Sample pushing FRET peaks for Pif1 pushing Cy5-SSB on 3′dT-Cy3-(dT)140 at 50 μM ATP. (C) Sample pushing FRET peaks for Rep pushing Cy5-SSB on 5′dT-Cy3-(dT)140 at 5 mM ATP. (D) Sample pushing FRET peaks for UvrD pushing Cy5-SSB on 5′dT-Cy3-(dT)140 at 5 mM ATP. (E) Sample FRET fluctuations for Cy5-SSB diffusing on 3′dT-Cy3-(dT)140. Solution conditions: 30 mM Tris⋅HCl, pH 8.1, 100 mM NaCl, 5 mM MgCl2, 1 mM DTT, 0.1 mg/mL BSA, 0.5% (wt/vol) dextrose, 3 mM Trolox, 1 mg/mL glucose oxidase, and 0.4 mg/mL catalase, 25 °C. Green, Cy3 fluorescence; red, Cy5 fluorescence; blue, FRET efficiency calculated from Cy3 and Cy5 emission intensities.