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. 2016 May 16;113(22):6242–6247. doi: 10.1073/pnas.1605957113

Fig. 1.

Fig. 1.

cysLT-dependent submucosal swelling and mucin release elicited by Alternaria. (A) Representative histological coronal section through the incisors of the mouse skull of a PBS-challenged mouse with CAE stain (50× magnification). (B) A 630× magnification of Inset in A, rotated 90°. Black arrowheads point to MCs. (C–E) PAS staining of nasal septum 1 h after i.n. administration of (C) PBS or (D and E) 30 µg of Alternaria (magnification: 630×). (Scale bar, 20 µm.) Black arrows indicate mucin-containing GCs. Red arrows indicate GCs with partial extrusion of mucin. (F and G) WT (black bars) and Ltc4s−/− (gray bars) mice. (F) Submucosal swelling, indicated by the difference in the submucosal area between PBS (0 µg) and Alternaria-treated (30 µg) mice. (G) Mucin release, indicated by the difference in the total number of PAS+ cells detected between PBS and Alternaria-treated mice. Results are means ± SEM pooled from four independent experiments with the number of mice per group indicated on each bar in parentheses. *P < 0.05, **P < 0.01.