Table 1.
Data collection, phasing and refinement statistics
| Data collection and statistics | Sel Met (apo) | CMP complex |
| Data collection | ||
| Space group | P212121 | C2221 |
| Cell dimensions | ||
| a, b, c (Å) | 82.93, 82.93, 120.71 | 92.75, 159.39, 120.04 |
| Resolution (Å) | 50–2.3 (2.36–2.3)* | 50–2.1 (2.15–2.10) |
| Rsym | 0.073 (0.93) | 0.094 (0.64) |
| I/σI | 19.1 (2.2) | 13.3 (2.85) |
| Completeness (%) | 99.8 (100) | 100 (100) |
| Redundancy | 5.8 (5.8) | 7.2 (7.5) |
| Refinement | ||
| Resolution (Å) | 41.5–2.3 | 50–2.1 |
| No. reflections | 37,670 | |
| Rwork/Rfree | 0.186/0.229 | 0.169/0.211 |
| No. atoms | ||
| Protein | 6,211 | 6,187 |
| Ligand/ion | 1 | 42 |
| Water | 562 | 836 |
| B-factors | ||
| Protein | 33.2 | 24.1 |
| Ligand/ion | 42.9 | 11.6 |
| Water | 34.3 | 31.7 |
| Rms deviations | ||
| Bond lengths (Å) | 0.003 | 0.005 |
| Bond angles (°) | 0.636 | 0.852 |
A single crystal was used in each case to determine the structure.
*
Values in parentheses are for highest-resolution shell.