Fig. 6.

SP110 isoform expression by genotype at cfa25:45,444,053. Expression levels of the full-length (A; FL) and Δ exon 8 (B; Δex8) transcripts measured in the total RNA purified from blood of healthy NSDTRs genotyped for SNP cfa25:45,444,053 are shown. Twenty-three dogs homozygous for the G allele, 46 heterozygotes for the G and T alleles, and 43 homozygotes for the T allele were analyzed. Red boxes (risk), black boxes (heterozygotes), and blue boxes (nonrisk) represent the 25–75% interquartile range with the median, and the 10–90 percentile range with maximum and minimum values. The gene expression was normalized to the levels of the housekeeping gene TBP and analyzed using one-way ANOVA. The full-length transcript is down-regulated in the risk, whereas the Δ exon 8 transcript is up-regulated.