Effects of suppression of MITF expression in C8161.9 cells. (A) Expression of MITF, BPTF, BCL2, BCL-XL, and CCND2 RNA following stable expression of anti-MITF shRNA #22 in C8161.9 cells. (B) Expression of MITF, BPTF, BCL2, BCL-XL, and CCND2 protein following stable expression of anti-MITF shRNA #22 in C8161.9 cells. (C) Assay of C8161.9 colony formation following suppression of MITF expression. (D) Effects of overexpression of BPTF cDNA or vector control on colony forming capacity of C8161.9 cells expressing anti-MITF shRNA. In A, C, and D data presented reflect mean ± SEM of three replicates. *P < 0.05.