Fig. 2.

KDM5D as a potential mediator of docetaxel sensitivity with DHT stimulation. (A) Heat map of RNA expression level (logtwofold change by 10 nM DHT) based on RNA-seq data with LNCaP and LAPC4 after 4- and 24-h DHT induction. The mean expression value of three independent experiments is presented. (B) Volcano plot of 236 genes (consisting of GO:0016573 histone acetylation, GO:0016575 histone deacetylation, GO:0016571 histone methylation, and GO:0016577 histone demethylation). The mean expression value of three independent experiments was used for the analysis. (C) LNCaP or LAPC4 was transfected with siRNA of the indicated targets (based on the expression level of the relevant gene) and negative control (50 nM) 2 d before treatment with docetaxel. Transfected cells were treated with varied docetaxel concentrations in 10% CSS media supplemented with 10 nM DHT for 6 d. GI₅₀ of docetaxel was determined after 6 d of treatment. Results are expressed as mean ± SEM. (D) LNCaP and DU145 were transfected with 50 nM si-K (KDM5D) and si-C (control) 2 d before docetaxel treatment. Transfected cells were treated with varied docetaxel concentrations in 10% CSS media with and without 10 nM DHT for 6 d. GI₅₀ of docetaxel was determined after 6 d of treatment. Results are expressed as mean ± SEM. Nuclear fractions were collected 48 h after the indicated siRNA transfection and subjected to immunoblotting with the indicated antibodies.