Fig. 3.

KDM5D and AR in the nucleus cooperate in rendering docetaxel sensitivity. (A) PC3-pLenti-control and PC3-pLenti-KDM5D cells were transfected with the indicated AR constructs (AR-FL and AR-v7) using a forward transfection protocol. The next day, cells were plated in a 96-well plate in CSS with and without 10 nM DHT, followed by the indicated treatment (10 nM docetaxel) for 6 d. Inhibitory effect on cell growth is presented as a relative value (mean ± SEM) compared with control as 100%. (B) Transfected cells were starved in CSS for 48 h, followed by treatment with and without 10 nM DHT for 24 h. Nuclear fractions were collected and subjected to immunoblotting with the indicated antibodies.