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. 2016 May 16;113(22):6259–6264. doi: 10.1073/pnas.1600420113

Fig. S1.

Fig. S1.

(A) LNCaP and LAPC4 cells were cultured with and without 10 nM DHT in 10% CSS media. Twenty-four hours after DHT induction, whole-cell lysates were collected and subjected to immunoblotting with the indicated antibodies. (B) LNCaP and LAPC4 cells were treated with the indicated concentrations of docetaxel in 10% CSS media with and without 10 nM DHT for 6 d. The inhibitory effect on cell growth by docetaxel is presented as a relative value (mean ± SEM) compared with control as 100%. (C) LNCaP and LAPC4 cells were treated with and without 10 nM docetaxel in 10% CSS media with and without 10 nM DHT. After 48-h treatment, whole-cell lysates were collected and subjected to immunoblotting with the indicated antibodies. (D) Cells were treated with the indicated concentrations of enzalutamide in 10% CSS media supplemented with 10 nM DHT for 96 h. The inhibitory effect on cell growth is presented as a relative value (mean ± SEM) compared with control as 100%. (E) LAPC4 cells were treated with and without 10 μM enzalutamide in 10% CSS media with and without 10 nM DHT. After 24-h treatment, whole-cell lysates were collected and subjected to immunoblotting with the indicated antibodies.