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. 2016 May 16;113(22):6307–6312. doi: 10.1073/pnas.1602757113

Fig. S2.

Fig. S2.

The adhesion, hydration, and germination of vps41 pollen grains on the stigma. (A and B) Hydration of vps41/gVPS41-GFP pollen (A) and vps41 pollen grains (B) on the emasculated wild-type stigma. Both the complemented pollen and vps41 pollen expand in minutes after pollination on the stigma surface. The examples depicted here are representative of similar observations of 30 pollen grains. (C–F) SEM images of vps41/gVPS41-GFP pollen (C and D) and vps41 pollen grains (E and F) on the emasculated wild-type stigma. After germination on the stigma, the complemented pollen tubes penetrate into the papillar cell wall before traversing down to the base of the cell. Additionally, vps41 pollen is indistinguishable from the complemented pollen in behavior on the stigmatic surface. The examples depicted here are representative of similar observations of 20 pollen grains.