Fig. S1.

Sequence alignment of a representative set of type I and II HEs. The membrane-proximal domain is indicated in red, the esterase domain in green, and the lectin domain in blue. The type I/II distinctive elements, i.e., the α1β2 cysteine-loop (orange circles), the Ala-Ser substitution (red arrowhead), and the β16α6 segment (green circles) are indicated. Catalytically important residues are annotated in red below. A comprehensive alignment of all published HE sequences that was used to identify consistent differences between type I and II esterases is available upon request.