Table 1.

TnSeq screening results of S. aureus Himar1 transposon mutant library in epithelial cells

Mutant*	P value†	Product
Inactivated genes enriched in intracellular S. aureus‡
ssr42	<10−6	Small stable RNA (SSR) 42
rsp	0.0004	AraC-type transcriptional regulator
geh	0.037	Glycerol ester hydrolase
ruvA	0.045	Holliday junction DNA helicase RuvA
hemL	0.050	Glutamate-1-semialdehyde aminotransferase
Inactivated genes depleted in intracellular S. aureus
guaA	0.0003	Bifunctional GMP synthase
0220	0.0004	Transmembrane efflux pump protein, putative
pbuX	0.0004	Xanthine permease, putative
purM	0.003	Phosphoribosylformyl glycinamidine cyclo-ligase PurM
1920	0.008	ATP-dependent RNA helicase, DEAD box family, putative
2160	0.008	Phosphosugar-binding transcriptional regulator, putative

^*Gene or locus IDs according to NCBI GenBank accession no. CP006706.1 (i.e., 0181 represent RSAU_000181).

^†The P values were corrected for multiple testing and genes/loci showing P < 0.05 were reported as significantly increased or decreased. For further details, see SI Appendix, Table S1.

^‡Trend followed by the mutant in the given genes/loci throughout the intracellular passages of screening.