Fig. 6.

Conversions of MeCLA to MeCLA + 16 Da (MeCLA+16) by recombinant LBO protein synthesized in E. coli. MeCLA was incubated with LBO protein for 15 min, and the extracts were analyzed using electrospray ionization (ESI)-positive mode liquid chromatography–MS/MS. (A) Selected reaction monitoring chromatograms for MeCLA-fed LBO (Upper) without or (Lower) with 2-oxoglutarate. (B) Full-scan spectra of product ions from (Upper) substrate MeCLA and (Lower) metabolite MeCLA+16. The retention times of MeCLA and MeCLA+16 are 15.9 and 8.7 min, respectively.