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SDS/PAGE of recombinant LBO protein. Soluble protein fractions in E. coli having an LBO expression vector (LBO) and empty vector (EV) were fractionated using an affinity column for histidine-tagged proteins and then, loaded in a 4–12% (wt/vol) polyacrylamide gradient gel. The gel was stained using Coomassie Brilliant Blue. M, protein marker.
