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Consumption of CL, CLA, and MeCLA by recombinant LBO protein. Empty vector-transformed cells were used as a control. Substrates were incubated with soluble protein fractions prepared from E. coli having either (Upper) empty vector or (Lower) LBO, and the extracts were analyzed using liquid chromatography–MS/MS. Selected Reaction Monitoring chromatograms of (Left) CL, (Center) CLA, and (Right) MeCLA are shown.
