Table 3. Sequences of primer pairs used for PCR to detect HBV genome.
Gene and primer a | Sequence (5′ 3′) | Nucleotide position |
---|---|---|
Surface gene | ||
P1s | TCACCATATT CTTGGGAACA AGA | 2823-2845 |
S1–2a | CGAACCACTG AACAAATGGC | 685-704 |
Core gene | ||
C1s | CTGGGAGGAG TTGGGGGA | 1730-1747 |
C2a | GTAGAAGAAT AAAGCCC | 2503-2487 |
C3s | GGTCTTTGTA CTCGGAGGCT G | 1763-1783 |
C4a | ATACTAACAT TGACATTCCC | 2455-2436 |
X gene | ||
X1s | CTAGCCGCTT GTTTTGCTCG | 1282-1301 |
X2a | TTATGCCTAC AGCCTCCTAG | 1666-1647 |
X3s | GGTCTTACAT AAGAGGACTC | 1518-1537 |
X4a | GTTCACGGTG GTCTCCAT | 1625-1608 |
a s, sense; a, antisense