Figure 2. Immunogenicity of esx constructs compared. (A) Immunization schedule for DNA. Mice (n = 5 per group) were vaccinated plus EP with each individual DNA esx construct 3 times at 2-wk intervals and spleens were harvested 1 wk after last immunization to analyze the cellular immune responses (B–F). Esx-specific T cell responses in (B–F) were measured against a pool of peptides to their respective antigens by IFN-γ ELISpot. Error bars indicate SEM and experiments were performed independently at least 2 times with similar results.