Figure 4. The NGcGM3/VSSP vaccine increases the maturation of Bone Marrow-derived plasmacytoid Dendritic cells. BM of C57BL/6 mice were differentiated in FLT3L-enriched medium. B220⁺ purified cells were cultured overnight in the presence of LPS (0.5 µg/mL), CpG-A (5 µg/mL), NGcGM3/VSSP (Vac 0.1 µg/mL), or medium (im-pDC). (A) Frequency of CD86⁺B220⁺ cells. (B) IFNα secretion by NGcGM3/VSSP treated pDC in culture. Data are representative of 2 independent experiments. (C) C57BL/6 mice were injected ip with the NGcGM3/VSSP vaccine and serum levels of IFNα, IL6 and IL12p40 were determined by ELISA at 2-h intervals.