Figure 2. Neutrophil transwell migration during AKI.
Rhabdomyolysis-induced AKI causes impaired neutrophil transwell migration as early as 4h after induction (A, n=7–8), lasting at least as long as 24h (B, n=6–9). Nephrotoxic AKI (n=6) significantly inhibits neutrophil transwell migration 24h after induction (B). Incubation in normal serum completely restores normal neutrophil transwell migration in rhabdomyolysis-induced AKI (n=8–10) but only incompletely and nephrotoxic AKI (n=8) (C). Normal neutrophil transwell migration is completely restored in both nephrotoxic AKI (n=6) and rhabdomyolysis-induced AKI (n=8–10) after incubation in MEM buffer (D).