Fig. 8.

Pleural inflammation and lavage neutrophilia is increased in PAI-1 deficiency after induction of S. pneumoniae mediated pleural injury. Pleural injury was induced in WT and PAI-1^−/− mice by intrapleural injection of S. pneumoniae and maintained for 3 days. CT scan and pulmonary function analyses were performed 3 days after infection. a S. pneumoniae infection induced significant pleural injury in PAI-1^−/− mice (volume and compliance, p = 0.02 and p = 0.01, respectively). b Pleural lavage fluid from S. pneumoniae infected PAI-1^−/− mice contained a significantly higher percentage of neutrophils than identically treated WT mice. Neutrophil percentage was determined by differential staining and cellular differential performed at 100× oil magnification, n = 4–7/group. c Tissue sections from saline and S. pneumoniae-infected mice were prepared from the lungs of WT and PAI-1^−/− mice after 3 days. The sections were then stained for fibrin (ogen) (red) by IHC. All images were taken at 20× magnification. Images are representative of 30 fields/slide and n = 4–7 mice/group. Pleural lavages (PL) were performed on S. pneumoniae infected mice at 3 days