Figure 2.

Establishment of mouse models with MPO-ANCA production. Experimental protocol (A). BALB/c, NZW, B6/N, B6/J, and DBA mice (14-week-old female) were given i.p. injection of PMA (50 ng at days 0 and 7) and oral PTU (2.5 mg/day) for 4 weeks (n = 5/strain, p.o.: Per Os). Mouse urine was collected during the last 24 h using metabolic cages, and then hematuria was assessed by dipsticks. Blood samples were obtained at days 14 and 28, and then the concentrations of BUN and Cr were determined. The lungs, kidneys, and peritoneal tissues were obtained at day 28. Serum titers of MPO-ANCA determined by ELISA were shown with the results of the urinalysis and concentrations of Cr in the serum (B). The normal limit of serum Cr level is 0.4 mg/dl.