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. 2016 Jun 8;7:800. doi: 10.3389/fpls.2016.00800

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Copyright © 2016 de Jonge, Kodde, Severing, Bonnema, Angenent, Immink and Groot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Relative expression of BoSTM (orthologous to STM in Arabidopsis thaliana) at day 5 after finishing the cold treatment to induce blindness. Expression levels measured by Q-PCR using meristem enriched tissue from non-blind looking seedlings (Sensitive non-blind) and blind looking seedlings (Sensitive blind) from the sensitive genotype (line AG5010) and from non-blind seedlings (Resistant non-blind) from a resistant genotype (line AG1020). Error bars represent the standard error of four biological replicates. (B) Expression of BoSTM in seedlings of different phenotype at day 10 after the cold induction of seeds from line AG5010 (a sensitive genotype). ‘Normal’ represents the expression of plants with a meristem. ‘Blind with axillary shoots’ represents plants that lost their SAM but develop side shoots in the axils of the cotyledons. ‘Blind with leaf like structure’ represent the expression of plants that produced one leaf-like structure before arresting the meristem. ‘Blind only cotyledons,’ represents plants that only developed cotyledons and no side-shoots. Expression is provided in comparison to the resistant non-blind seedlings. Error bars represent the standard error of four biological replicates.