Table 3.
Cecal concentrations of SCFAs (n = 10) and fecal dry matter (n = 20) and heat value (n = 20) measured 8 h after the last dose in Experiment B.
| Group | HF |
HF B. thet. |
HF B. thet. lactitol |
HF B. thet. PDX |
||||
|---|---|---|---|---|---|---|---|---|
| Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |
| Cecal SCFAs (μmol/g) | ||||||||
| Acetic acid | 47.51 | 2.42 | 51.28 | 2.53 | 21.18*** | 1.93 | 22.83*** | 1.99 |
| Propionic acid | 11.95 | 0.64 | 12.81 | 0.8 | 10.85 | 1.14 | 9.39 | 0.59 |
| Butyric acid | 3.63 | 0.61 | 4.02 | 0.88 | 2.74 | 0.46 | 1.92 | 0.34 |
| Lactic acid | 0.31 | 0.17 | 0.69 | 0.2 | 0.9 | 0.2 | 0.43 | 0.28 |
| Cecal BCFAs (μmol/g) | ||||||||
| Isobutyric acid | 0.43 | 0.05 | 0.52 | 0.05 | <0.1*** | 0.02 | <0.1*** | 0.02 |
| 2-methylbutyric acid | 0.16 | 0.02 | 0.19 | 0.03 | <0.1* | 0.03 | 0.1 | 0 |
| Isovaleric acid | 0.16 | 0.03 | 0.19 | 0.03 | <0.1*** | 0.01 | <0.1*** | 0.01 |
| Sum of BCFAs (μmol/g) | 0.75 | 0.1 | 0.9 | 0.1 | 0.13*** | 0.03 | 0.14*** | 0.03 |
| Sum of all SCFAs (μmol/g) | 64.5 | 3.29 | 69.85 | 3.69 | 35.99*** | 2.93 | 35.18*** | 2.5 |
| Fecal dry matter (%) | 77.05 | 1.76 | 76.47 | 1.9 | 62.26*** | 2.12 | 57.68*** | 2.08 |
| Fecal heat value (KJ/g dry matter) | 16.68 | 0.22 | 18.38*** | 0.2 | 16.00 | 0.26 | 17.30 | 0.19 |
SCFAs are expressed as μmol/g wet weight. *p < 0.05, **p < 0.01, and ***p < 0.001, compared to the HF control group (ANOVA). BCFA, branched-chain fatty acid; B. thet., Bacteroides thetaiotaomicron; HF, high-fat diet; PDX, polydextrose; SCFA, short-chain fatty acid; SEM, standard error of the mean.