FIGURE 3.

Cholesterol-enriched diet increases the accumulation of phosphorylated tau in endolysosomes. (A) In muscle fibers from control rabbits, SMI-31 positive staining was weak, but in muscle fibers from cholesterol-fed rabbits SMI-31 positive staining appeared as large aggregates (40X). (B) SMI-31 positive staining of phosphorylated tau (red) co-distributed with EEA1-positive staining of endosomes (green) and LAMP2-positive staining of lysosomes (green) in muscle from cholesterol-fed rabbits. Bar = 20 μm. (C) Representative western blots were shown. Rabbits fed cholesterol-enriched diet (n = 9) exhibited significantly (^∗p < 0.05) increased protein levels of phosphorylated tau in skeletal muscle, when compared with controls (n = 5).