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. 2016 Jun 8;8:129. doi: 10.3389/fnagi.2016.00129

FIGURE 4.

FIGURE 4

Cholesterol-enriched diet increases the accumulation of ubiquitin in endolysosomes. (A) In muscle fibers from control rabbits, ubiquitin positive staining was weak, but in muscle fibers from cholesterol-fed rabbits ubiquitin positive staining appeared as large aggregates (40X). (B) Ubiquitin positive staining (red) co-distributed with EEA1-positive staining of endosomes (green) and with LAMP2-positive staining of lysosomes (green) in muscle from cholesterol-fed rabbits. Bar = 20 μm. (C) Representative western blots were shown. Rabbits fed cholesterol-enriched diet (n = 9) exhibited significantly (∗∗∗P < 0.001) increased protein levels of ubiquitin in skeletal muscle, when compared with controls (n = 5).