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. 2016 Jun 8;8:129. doi: 10.3389/fnagi.2016.00129

FIGURE 6.

FIGURE 6

Cholesterol-enriched diet increases deposition of Aβ in endosomes and autophagosomes. (A) In muscle fibers from control rabbits, 4G8 positive Aβ signals were weak, but in muscles from cholesterol-fed rabbits 4G8 positive Aβ signals appeared as large aggregates inside muscles (40X). (B) Representative western blots were shown. Cholesterol-enriched diet (n = 9) increased significantly protein levels of Aβ oligomers (∗p < 0.05) in skeletal muscle, when compared with controls (n = 5). (C) 4G8-positive staining of Aβ (red) co-distributed with EEA1 positive staining of endosomes (green) in skeletal muscle from cholesterol-fed rabbits. Bar = 20 μm. (D) In muscle fibers from control rabbits, LC3 positive autophagosome signals were weak and diffuse, but appeared as large aggregates inside muscles from cholesterol-fed rabbits (40X). (E) Representative western blots were shown. Cholesterol-enriched diet (n = 9) increased significantly protein levels of LC3-II (∗∗p < 0.01) in skeletal muscle, when compared with controls (n = 5). (F) 4G8-positive staining of Aβ (red) co-distributed with LC3-positive staining for autophagosomes (green) in skeletal muscle from cholesterol-fed rabbits. Bar = 20 μm. (G) EEA1positive endosomes (green) co-distributed with LC3-positive staining for autophagosomes (red) in skeletal muscle from cholesterol-fed rabbits. Bar = 20 μm.