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. 2016 Jun 8;7:879. doi: 10.3389/fmicb.2016.00879

FIGURE 2.

FIGURE 2

Inhibition of ROS production causes a reduction of NETs formation C. albicans induced. PMNs, pre-treated or not with N-acetyl L Cysteine (NAC, 1mM), were incubated without stimuli (NS) or with C. albicans hyphae (CAH), live yeast (CALY) or heat inactivated yeast (CAIY), and ROS production (A) and NETs formation (B) were determined as described in Section “Materials and Methods.” (A) The lines represent the kinetic of ROS release. The bars represent the total amounts of produced ROS obtained by analyzing the area under the curve (AUC). Lines are representatives of n = 5 experiments with similar results. Bars are the mean ± SEM of n = 5 experiments with similar results. ∗P < 0.05 PMNs NAC pre-treated vs. PMNs not pre-treated. (B) NET formation in response to CAH, to CALY or to CAIY after 15 min and 4 h of incubation. Bars are the mean ± SEM of n = 7 experiments with similar results. ∗P < 0.05 PMNs pre-treated with NAC + CAH or CALY vs. PMNs not pre-treated; #P < 0.05 PMNs + CAIY vs. PMNs NS.